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Arizona State University Biophysics Seminar - Monarin Uervirojnangkoorn

Category: Seminar
Description:

Prime: Post-Refinement and Merging Program for XFEL Data

Abstract

An X-ray free-electron laser (XFEL) delivers a micron-size beam with high brilliance femtosecond pulses. It has been shown that XFEL crystallography can produce diffraction data without radiation damage. Moreover, XFELs may enable data collection on crystals too small for current micro-focus synchrotron beamlines. A major unresolved challenge is how to best process XFEL data – due to the “diffract-and-destroy” characteristic of the experiment, only “still” diffraction images can be recorded. Moreover, small crystals are often destroyed by a single pulse, and data are often measured from many crystals in random orientations. Thus, the still diffraction data may vary in terms of unit-cell dimensions and crystal mosaicity, as well as shot-to-shot differences in the energy spectrum arising from the SASE process. Ideally, these variations need to be taken into account during data integration and merging. We have implemented a computer program, prime (post-refinement and merging). We tested the program with XFEL data sets and observed significant improvements in terms of the quality of the diffraction data and quality of the refined model.

When: Wednesday, October 1 2014, 3:30 PM - 4:30 PM (ET) (UTC -05:00) Eastern Time (US & Canada), Bogota, Lima
Where: Arizona State University - 487 Goldwater Center, Tempe, AZ 85287
Website: https://physics.asu.edu/seminar/biophysics-seminar/2014/10/01/prime-post-refinement-and-merging-program-xfel-data
Tags:
  1. data
  2. xfel