Home (current)

  • Scientists who make movies of molecules in motion have a new high-speed camera to shoot with. The €1.2-billion (US$1.4-billion) European X-ray Free Electron Laser (XFEL) will start running its first experiments in September near Hamburg, Germany. The European XFEL fires powerful X-rays in bursts of a few hundred femtoseconds: so short that, like st...
  • Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) ...
  • BioXFEL researcher Peter Schwander released a publication, along with his peers, to Nature Methods Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our result...
  • A research collaboration led by the University of Wisconsin-Milwaukee has for the first time created a three-dimensional movie showing a virus preparing to infect a healthy cell. The research has the potential to fundamentally advance our understanding of how biological processes inside the cell work. That could lead to better treatment for the ho...
  • BioXFEL scientist Vadim Cherezov and his colleagues released a new publication to CellPress: X-ray free electron lasers (XFELs) have the potential to revolutionize macromolecular structural biology due to the unique combination of spatial coherence, extreme peak brilliance, and short duration of X-ray pulses. A recently emerged serial femtosecond ...
  • BioXFEL scientist Richard A. Kirian, along with his peers, published a research article onto IUCrj: This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono Riv...
  • BioXFEL scientist Marc Messerschmidt, along with his colleagues, published a research article in OSA publishing. Summary: Determining fluctuations in focus properties is essential for many experiments at Self-Amplified-Spontaneous-Emission (SASE) based Free-Electron-Lasers (FELs), in particular for imaging single non-crystalline biological partic...
  • BioXFEL scientist Vadim Cherezov, along with others, publishes research article in ScienceDirect. Abstract: The nicotinamide nucleotide transhydrogenase (TH) is an integral membrane enzyme that uses the proton-motive force to drive hydride transfer from NADH to NADP+ in bacteria and eukaryotes.
  • Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs.
  • A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues–Frank space, in which all crystal systems can be represented without singularities.
  • Cytochrome c oxidase (CcO), the terminal enzyme in the electron transfer chain, translocates protons across the inner mitochondrial membrane by harnessing the free energy generated by the reduction of oxygen to water.
  • Understanding the 3D molecular structure of important nano-objects like proteins and viruses is crucial in biology and medicine. With recent advances in X-ray technology, scientists can now collect diffraction images from individual particles, ultimately allowing researchers to visualize molecules at room temperature.

Latest @BioXFEL

  • The BioXFEL Center is pleased to announce that our very own Dr. Abbas Ourmazd of the University of Wisconsin-Milwaukee is an author on two joint papers that were included in the background of the recent Nobel Prize in Chemistry using Cryo-EM to determine biological structure.  These papers concerned conformational movies and energy landscapes, whic...
  • On September 20th, Hurricane Maria made landfall over Puerto Rico and devastated the island. Weeks later, communications and basic necessities have still not been restored. It will likely take months to restore a sense of normalcy and years to fully recover. In response to this disaster, BioXFEL has created 2 new programs to assist our friends a...
  • Designing molecules that selectively bind to a specific receptor type is often challenging, but can be crucial for different therapeutic purposes. Both angiotensin II receptors are important drug targets, since the blockade of AT1R has anti-hypertensive effects, while the modulation of AT2R could be useful for cardioprotection, neuropathic pain rel...
  • Yun-Xing Wang in collaboration with BioXFEL Researchers, publishes new article "Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography" in Nature. 
  • BioXFEL member and undergraduate researcher, Fiacre Kabayiza, has created a game to help educate school-aged students on the topic of XFEL science. Employing a simple, yet engaging, tap-based gameplay and a time-proven minimal 8-bit graphical art style, he delivers a game that introduces tweens and teens to XFELs, structure determination, and the s...
  • X-ray crystallography is a technique that can be used by scientists to map the 3-dimensional physical space of proteins and other biological molecules to an atomic level of detail. Over the last four decades or so, this technique has been used to map over 100,000 protein structures that can be found in the publicly accessible Protein Data Bank, wit...

Mailing List

Resources

Video Library

Events

News

Publications