Reconstructing three-dimensional helical structure with an X-ray free electron laser
Category
Published on
Type
journal-article
Author
M. Uddin
Citation
Uddin, M., 2016. Reconstructing three-dimensional helical structure with an X-ray free electron laser. Journal of Applied Crystallography, 49(2), pp.450–456. Available at: http://dx.doi.org/10.1107/s1600576716000303.
Abstract
Recovery of three-dimensional structure from single-particle X-ray scattering of completely randomly oriented diffraction patterns as predicted a few decades ago has been realized owing to the advent of the new emerging X-ray free electron laser (XFEL) technology. Since the world's first XFEL started operation in June 2009 at SLAC National Laboratory at Stanford, the first few experiments have been conducted on larger objects such as viruses. Many of the important structures of nature such as helical viruses or DNA consist of helical repetition of biological subunits. Hence development of a method for reconstructing helical structure from collected XFEL data has been a top research priority. This work describes the development of a method for solving helical structures such as tobacco mosaic virus from a set of randomly oriented simulated diffraction patterns exploiting the symmetry and Fourier space constraint of the diffraction volume.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
