Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals
Category
Published on
Type
journal-article
Author
Salah Awel and Richard A. Kirian and Max O. Wiedorn and Kenneth R. Beyerlein and Nils Roth and Daniel A. Horke and Dominik Oberthür and Juraj Knoska and Valerio Mariani and Andrew Morgan and Luigi Adriano and Alexandra Tolstikova and P. Lourdu Xavier and Oleksandr Yefanov and Andrew Aquila and Anton Barty and Shatabdi Roy-Chowdhury and Mark S. Hunter and Daniel James and Joseph S. Robinson and Uwe Weierstall and Andrei V. Rode and Saša Bajt and Jochen Küpper and Henry N. Chapman
Citation
Awel, S. et al., 2018. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Journal of Applied Crystallography, 51(1), pp.133–139. Available at: http://dx.doi.org/10.1107/s1600576717018131.
Abstract
High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
