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X-ray and UV radiation-damage-induced phasing using synchrotron serial crystallography

By Nicolas Foos, Carolin Seuring, Robin Schubert, Anja Burkhardt, Olof Svensson, Alke Meents, Henry Chapman1, Max H. Nanao

1. Center for Free-Electron Laser Science

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Category

BioXFEL Publications

Published on

Type

journal-article

Author

Nicolas Foos and Carolin Seuring and Robin Schubert and Anja Burkhardt and Olof Svensson and Alke Meents and Henry N. Chapman and Max H. Nanao

Citation

Foos, N. et al., 2018. X-ray and UV radiation-damage-induced phasing using synchrotron serial crystallography. Acta Crystallographica Section D Structural Biology, 74(4), pp.366–378. Available at: http://dx.doi.org/10.1107/s2059798318001535.

Abstract

Specific radiation damage can be used to determine phases de novo from macromolecular crystals. This method is known as radiation-damage-induced phasing (RIP). One limitation of the method is that the dose of individual data sets must be minimized, which in turn leads to data sets with low multiplicity. A solution to this problem is to use data from multiple crystals. However, the resulting signal can be degraded by a lack of isomorphism between crystals. Here, it is shown that serial synchrotron crystallography in combination with selective merging of data sets can be used to determine high-quality phases for insulin and thaumatin, and that the increased multiplicity can greatly enhance the success rate of the experiment.

DOI

http://dx.doi.org/10.1107/s2059798318001535