Time-resolved Crystallography on Protein Photoreceptors and Enzymes
Category
Published on
Type
book-chapter
Author
Marius Schmidt
Citation
Schmidt, M. (2023). Time-resolved Crystallography on Protein Photoreceptors and Enzymes. Integrated Structural Biology, 203–240. https://doi.org/10.1039/bk9781837670154-00203
Abstract
Time-resolved macromolecular crystallography (TRX) unifies structure determination of biomolecules with chemical kinetics. Developed at synchrotron light sources, TRX has been transformed by serial femtosecond crystallography (SFX) at X-ray free electron laser (XFEL) facilities. With TR-SFX, femtosecond time-resolution is possible and cyclic and non-cyclic macromolecular reactions can be equally well investigated. Here, progress in the field is illustrated by findings from several successful experiments on photoreceptors and enzymes at XFELs and at synchrotron X-ray sources.
