Direct phasing in femtosecond nanocrystallography. II. Phase retrieval
Category
Published on
Type
journal-article
Author
Joe P. J. Chen and John C. H. Spence and Rick P. Millane
Citation
Chen, J.P.J., Spence, J.C.H. & Millane, R.P., 2014. Direct phasing in femtosecond nanocrystallography. II. Phase retrieval. Acta Cryst Sect A Found Adv, 70(2), pp.154–161. Available at: http://dx.doi.org/10.1107/s2053273313032725.
Abstract
X-ray free-electron laser diffraction patterns from protein nanocrystals provide information on the diffracted amplitudes between the Bragg reflections, offering the possibility of direct phase retrieval without the use of ancillary experimental diffraction data [Spenceet al.(2011).Opt. Express,19, 2866–2873]. The estimated continuous transform is highly noisy however [Chenet al.(2014).Acta Cryst.A70, 143–153]. This second of a series of two papers describes a data-selection strategy to ameliorate the effects of the high noise levels and the subsequent use of iterative phase-retrieval algorithms to reconstruct the electron density. Simulation results show that employing such a strategy increases the noise levels that can be tolerated.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
