Towards phasing using high X-ray intensity
Category
Published on
Type
journal-article
Author
Lorenzo Galli and Sang-Kil Son and Thomas R. M. Barends and Thomas A. White and Anton Barty and Sabine Botha and Sébastien Boutet and Carl Caleman and R. Bruce Doak and Max H. Nanao and Karol Nass and Robert L. Shoeman and Nicusor Timneanu and Robin Santra and Ilme Schlichting and Henry N. Chapman
Citation
Galli, L. et al., 2015. Towards phasing using high X-ray intensity. IUCrJ, 2(6), pp.627–634. Available at: http://dx.doi.org/10.1107/s2052252515014049.
Abstract
X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential `bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
