Shape transform phasing of edgy nanocrystals
Category
Published on
Type
journal-article
Author
J. P. J. Chen and J. J. Donatelli and K. E. Schmidt and R. A. Kirian
Citation
Chen, J.P.J. et al., 2019. Shape transform phasing of edgy nanocrystals. Acta Crystallographica Section A Foundations and Advances, 75(2), pp.239–259. Available at: http://dx.doi.org/10.1107/s205327331900113x.
Abstract
Diffraction patterns from small protein crystals illuminated by highly coherent X-rays often contain measurable interference signals between Bragg peaks. This coherent `shape transform' signal introduces enough additional information to allow the molecular densities to be determined from the diffracted intensities directly, without prior information or resolution restrictions. However, the various correlations amongst molecular occupancies/vacancies at the crystal surface result in a subtle yet critical problem in shape transform phasing whereby the sublattices of symmetry-related molecules exhibit a form of partial coherence amongst lattice sites when an average is taken over many crystal patterns. Here an iterative phase retrieval algorithm is developed which is capable of treating this problem; it is demonstrated on simulated data.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
