Polo: an open-source graphical user interface for crystallization screening
Category
Published on
Type
journal-article
Author
Ethan T. Holleman and Erica Duguid and Lisa J. Keefe and Sarah E. J. Bowman
Citation
Holleman, E.T. et al., 2021. Polo: an open-source graphical user interface for crystallization screening. Journal of Applied Crystallography, 54(2), pp.673–679. Available at: http://dx.doi.org/10.1107/s1600576721000108.
Abstract
Polo is a Python-based graphical user interface designed to streamline viewing and analysis of images to monitor crystal growth, with a specific target to enable users of the High-Throughput Crystallization Screening Center at Hauptman-Woodward Medical Research Institute (HWI) to efficiently inspect their crystallization experiments. Polo aims to increase efficiency, reducing time spent manually reviewing crystallization images, and to improve the potential of identifying positive crystallization conditions. Polo provides a streamlined one-click graphical interface for the Machine Recognition of Crystallization Outcomes (MARCO) convolutional neural network for automated image classification, as well as powerful tools to view and score crystallization images, to compare crystallization conditions, and to facilitate collaborative review of crystallization screening results. Crystallization images need not have been captured at HWI to utilize Polo's basic functionality. Polo is free to use and modify for both academic and commercial use under the terms of the copyleft GNU General Public License v3.0.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
