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Protocol for Structure Determination of SARS-CoV-2 Main Protease at Near-physiological-temperature by Serial Femtosecond Crystallography

By Fatma Betul Ertem, Omur Guven, Cengizhan Buyukdag, Oktay Gocenler, Esra Ayan, Busra Yuksel, Mehmet Gul, Gozde Usta, Barıs Cakılkaya, J. Austin Johnson, E. Han Dao, Zhen Su, Frederic Poitevin, Chun Hong Yoon, Christopher Kupitz1, Brandon Hayes1, Mengning Liang, Mark S. Hunter1, Alexander Batyuk, Raymond G. Sierra, Gihan Kaushylal Ketawala2, Sabine Botha2, Çağdaş Dağ, Hasan Demirci3

1. SLAC National Accelerator Laboratory 2. Arizona State University 3. Biosciences Division at SLAC National Accelerator Laboratory

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Category

BioXFEL Publications

Published on

Type

journal-article

Author

Fatma Betul Ertem and Omur Guven and Cengizhan Buyukdag and Oktay Gocenler and Esra Ayan and Busra Yuksel and Mehmet Gul and Gozde Usta and Barıs Cakılkaya and J. Austin Johnson and E. Han Dao and Zhen Su and Frederic Poitevin and Chun Hong Yoon and Christopher Kupitz and Brandon Hayes and Mengning Liang and Mark S. Hunter and Alexander Batyuk and Raymond G. Sierra and Gihan Ketawala and Sabine Botha and Çağdaş Dağ and Hasan DeMirci

Citation

Ertem, F. B., Guven, O., Buyukdag, C., Gocenler, O., Ayan, E., Yuksel, B., Gul, M., Usta, G., Cakılkaya, B., Johnson, J. A., Dao, E. H., Su, Z., Poitevin, F., Yoon, C. H., Kupitz, C., Hayes, B., Liang, M., Hunter, M. S., Batyuk, A., … DeMirci, H. (2022). Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography. STAR Protocols, 3(1), 101158. https://doi.org/10.1016/j.xpro.2022.101158

DOI

http://dx.doi.org/10.1016/j.xpro.2022.101158

Funding

NSF-STC Biology with X-ray Lasers (NSF-1231306)