Events: Details

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HWI Crystallization Workshop 2015 for BioXFEL Students and Postdoctoral Scholars

Category: Workshop

Sponsored in part by:

Please note agenda is subject to change.

Day 1 Lectures, Tuesday, June 2, 2015 – Flickinger Lecture Hall




8:30 - 9:00 am

Light breakfast in HWI atrium


9:00 - 9:05 am

Welcome to the Workshop

Ed Lattman

9:05 - 9:45 am

Fundamentals of crystal growth

Edward Snell

9:45 - 10:30 am

Strategies to sample crystallization space

Joe Luft

10:30 – 11:00 am

Coffee Break- HWI Atrium


11:00 - 11:45 am

Identifying crystal hits

Edward Snell

11:45 am -12:30 pm

Crystal Analysis - Applications of EM

Guillermo Calero

12:30 - 1:30 pm

Lunch- HWI Commons- 2nd Floor


1:30 - 2:15 pm

Practical Considerations for Data Analysis

Thomas Grant

2:15 - 3:00 pm

Optimizing hits - Small to large, large to small

Joe Luft

3:00 – 3:30 pm

Coffee Break- HWI Atrium


3:30 - 4:15 pm

Crystal properties than can bite

George Phillips

4:15 - 5:00 pm

Virus Crystallography, and Scaling Up to Batch

Rob Lawrence

5:00 - 5:45 pm

Plenary Lecture – Overview of Serial Crystallography

Petra Fromme

6:00 pm

Informal gathering at Buffalo’s world-famous Anchor Bar


 *  Workshop participants will register for specific workshops at the conclusion of Day 1

Day 2 Workshops, Wednesday, June 3, 2015 *                                              


Workshop Schedule A

Workshop Schedule B

8:30 - 9:00 am

Light breakfast in HWI atrium

9:00 - 10:00 am

Session 1 – HWI Classroom, 1st Floor

Session 2 – Open Lab Space, 2nd Floor

10:00 - 10:30 am

Coffee Break- HWI Atrium, 1st Floor

10:30 - 11:30 am

Session 3 – X-Ray Room, 3rd Floor

Session 5 – Open Lab Space, 3rd Floor

11:30 - 12:30 pm

Session 5 – Open Lab Space, 3rd Floor

Session 1 - HWI Classroom, 1st Floor

12:30 - 1:30 pm

Lunch- HWI Atrium, 1st Floor

1:30 - 2:30 pm

Session 2 – Open Lab Space, 2nd Floor

Session 3 - X-Ray Room, 3rd Floor

2:30 - 3:00 pm

Coffee Break- HWI Atrium, 1st Floor

3:00 - 4:30 pm

Session 4 - HWI Classroom, 1st Floor

Session 4 - HWI Classroom, 1st Floor

4:30 - 5:00 pm

Final workshop wrap up- Flickinger Lecture Hall

6:30 - 8:30 pm

Dinner with NSF Site Visit Participants-

Pearl Street Grill & Brewery

76 Pearl St, Buffalo, NY 14202
(716) 856-2337


ABYS Social Event - Location TBD

Session 1 - 1st Floor Classroom:   High-throughput sample screening, sample prep, imaging and data analysis. – Delivered by Joe Luft and Edward Snell, HWI High Throughput Laboratory (HTS)

  • Includes a virtual tour of Macroscope and a tour of the HTS lab
  • Students/postdocs will analyze their previously submitted samples in real time during the session (see below for details on sample submission)

Session 2 -  2nd Floor Lab :  Preparation and handling of nanocrystals – Delivered by Petra Fromme (ASU) and former member Chris Kupitz (UWM)

  • Members of the Fromme Lab at ASU and Chris Kupitz of UWM will cover practical considerations for the preparation, handling and delivery nanocrystals

Session 3 - 3rd Floor X-ray Room : The identification of nanocrystals – Delivered by  Edward Snell

  • Introduction and overview of the SONICC
  • Powder diffraction will be covered in depth and if time permits light scattering

Session 4 - 1st Floor Classroom : MacGyver Challenge the BioXFEL edition – Delivered by BioXFEL PI’s and Staff.

  • Use your improvisation and collaboration skills to develop a career-saving device

Session 5 - 3rd Floor Lab : Lab in a suitcase – Delivered by Alex Ros

  • Introduction to the techniques and equipment:
1) Microfluidic crystal sorting to reduce size heterogeneity of crystal suspensions
2) Droplet injection to reduce sample amount for crystallography with XFELs
3) Microfluidic crystal growth screening with the ‘Christmas Tree’ device
  • Attendees are encouraged to bring a nanocrystal suspensions to try crystal sorting with a microfluidic system


Crystallization Sample Submission Details

We will be offering our high-throughput crystallization screening services for free to all workshop participants. You will have the opportunity to submit your own sample, review your crystallization results in Session 1 of the workshop, and get advice and training in image and data analysis. This could be a great opportunity to find new crystallization conditions and have the results analyzed by our in-house crystallization experts. You do not have to submit a sample to participate in this session, but it will be a much more useful experience if you do.

We have 2 types of customized screens available, one for soluble proteins and one for membrane proteins.

Soluble screen details:

Reserve your spot before April 10
Ship your sample so that it arrives between April 14-16 or April 20-23
Soluble Protein Screen - Order and Submission Forms
This service is only free for workshop participants
Membrane screen details:
Reserve your spot before April 2
Ship your sample so that it arrives between April 7-9
Membrane Protein Screen - Order and Submission Forms
This service is free to all users until May 2015

If you are interested in using this service, please let me know as soon as you can and I can help arrange your submission. This will only be free if you attend the associated workshop session and we ask that you limit your samples to 1 per person for this event. If you have any further questions please contact Bill Bauer .


Travel expenses will be covered for students and postdocs attending the workshop. Rooms will be hard to find if you wait too long. The NHL combine is being held in Buffalo at the same time as the Site Visit. We have arranged discounted accommodations at two hotels which are within walking distance of HWI, bars, and restaurants.  You are able to begin booking your rooms now at the following locations:

1.  Double Tree by Hilton Buffalo Downtown

o   125 High Street, Buffalo, NY 14203

o   716-845-0112

o   Double Tree Buffalo Downtown

o   Group Name:  BioXFEL

o   Group Code:  BIO

o   $102 + tax

o   Cut-off date:  May 1st

o   6 min walk to HWI

2.  Comfort Suites

o   601 Main Street, Buffalo, NY 14203         

o   716-854-5500

o   Comfort Suites Downtown Buffalo

o   Please call to make reservation and mention Hauptman Woodward to get the discount

o   $79 + tax;  valet parking $10 per day

o   Cut-off date:  none

o   13 min walk to HWI

It would be advantageous to book rooms ASAP as there are only 30 rooms available at the Double Tree and the Comfort Suites is not holding any rooms it is strictly first come first served.

When: From Tuesday, June 2 2015 - 08:00 AM (ET) (UTC -05:00) Eastern Time (US & Canada), Bogota, Lima
To Wednesday, June 3 2015 - 5:00 PM (ET) (UTC -05:00) Eastern Time (US & Canada), Bogota, Lima
Contact: Bill Bauer
  1. Crystallopgraphy
  2. Data Analysis
  3. phase diagram
  4. powder diffraction
  5. XTAL Growth