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Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies

By David J. Aceti, Craig A. Bingman, Russell L. Wrobel, Ronnie O. Frederick, Shin-ichi Makino, Karl W. Nichols, Sarata C. Sahu, Lai F. Bergeman, Paul G. Blommel, Claudia C. Cornilescu, Katarzyna A. Gromek, Kory D. Seder, Soyoon Hwang, John G. Primm, Grzegorz Sabat, Frank C. Vojtik, Brian F. Volkman, Zsolt Zolnai, George Phillips1, John L. Markley, Brian G. Fox

1. Rice University

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Category

BioXFEL Publications

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Type

journal-article

Author

David J. Aceti and Craig A. Bingman and Russell L. Wrobel and Ronnie O. Frederick and Shin-ichi Makino and Karl W. Nichols and Sarata C. Sahu and Lai F. Bergeman and Paul G. Blommel and Claudia C. Cornilescu and Katarzyna A. Gromek and Kory D. Seder and Soyoon Hwang and John G. Primm and Grzegorz Sabat and Frank C. Vojtik and Brian F. Volkman and Zsolt Zolnai and George N. Phillips and John L. Markley and Brian G. Fox

Citation

Aceti, D.J. et al., 2015. Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies. Journal of Structural and Functional Genomics, 16(2), pp.67–80. Available at: http://dx.doi.org/10.1007/s10969-015-9198-1.

DOI

http://dx.doi.org/10.1007/s10969-015-9198-1