Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm
Category
Published on
Type
journal-article
Author
Hongxing He and Hengrui Fang and Mitchell D. Miller and George N. Phillips and Wu-Pei Su
Citation
He, H. et al., 2016. Improving the efficiency of molecular replacement by utilizing a new iterative transform phasing algorithm. Acta Crystallographica Section A Foundations and Advances, 72(5), pp.539–547. Available at: http://dx.doi.org/10.1107/s2053273316010731.
Abstract
An iterative transform method proposed previously for direct phasing of high-solvent-content protein crystals is employed for enhancing the molecular-replacement (MR) algorithm in protein crystallography. Target structures that are resistant to conventional MR due to insufficient similarity between the template and target structures might be tractable with this modified phasing method. Trial calculations involving three different structures are described to test and illustrate the methodology. The relationship of the approach toPHENIX Phaser-MRandMR-Rosettais discussed.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
