SFX Data Analysis Workshop at 2015 ACA

By Nadia Zatsepin1, Tom Grant2, Cornelius Gati

1. Arizona State University 2. Hauptman-Woodward Institute



Published on


Serial femtosecond crystallography has yielded several major and unique advances in structural biology previously unattainable with conventional technologies, including the potential for sub-picosecond time-resolved crystallographic studies, probing cyclic or even non-cyclic reactions. With the construction of more than a dozen new XFELs currently under way, and the multiple recent demonstrations of SFX at synchrotrons, the potential user base is growing significantly. The development and appropriate use of new software to tackle the unique problems of SFX data analysis is vital to making SFX practicable. 

The workshop will start with an introductory seminar, followed by most of the day dedicated to detailed hands-on tutorials with data sets collected at LCLS using the software suites Cheetah and CrystFEL, two of the most commonly used packages for SFX analysis. The concepts unique to SFX that we will be discussing are software independent.    

We will assume students have some basic knowledge of conventional crystallographic data analysis such as what indexing, integration, and merging are and will instead focus on the specific features of serial crystallography analysis. Practice data (from cxidb.org <http://cxidb.org> ) will be available. Students will be expected to bring laptops with appropriate software preinstalled. Students will be sent detailed information prior to the workshop. The workshop will begin at 8:30am and end at 5:00pm. Lunch will be provided.



In This Workshop

  1. Recent Advances in SFX at BioXFEL

    25 Jul 2015 | Teaching Materials | Contributor(s): John Spence

    Dr. John Spence, Scientific Director for BioXFEL, discusses recent advances in the field of serial crystallography.

  2. SFX Data Reduction and Preprocessing with Cheetah

    25 Jul 2015 | Teaching Materials | Contributor(s): Nadia Zatsepin

    Dr. Nadia Zatsepin discusses how to use the software program Cheetah to reduce raw SFX data from LCLS to a set of crystal "hits".

  3. SFX Indexing and Integration with CrystFEL

    25 Jul 2015 | Teaching Materials

    Dr. Thomas White discusses how to index and integrate serial crystallography images using the CrystFEL software package.