High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source
Category
Published on
Type
journal-article
Author
Jose M. Martin-Garcia and Lan Zhu and Derek Mendez and Ming-Yue Lee and Eugene Chun and Chufeng Li and Hao Hu and Ganesh Subramanian and David Kissick and Craig Ogata and Robert Henning and Andrii Ishchenko and Zachary Dobson and Shangji Zhang and Uwe Weierstall and John C. H. Spence and Petra Fromme and Nadia A. Zatsepin and Robert F. Fischetti and Vadim Cherezov and Wei Liu
Citation
Martin-Garcia, J.M. et al., 2019. High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. IUCrJ, 6(3), pp.412–425. Available at: http://dx.doi.org/10.1107/s205225251900263x.
Abstract
Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A2A adenosine receptor (A2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)