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Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

By Haoyuan Li, Reza Nazari1, Brian Abbey2, Roberto Alvarez, Andrew Aquila, Kartik Ayyer, Anton Barty, Peter Berntsen, Johan Bielecki, Alberto Pietrini, Maximilian Bucher, Gabriella Carini, Henry Chapman3, Alice Contreras, Benedikt J. Daurer, Hasan Demirci4, Leonie Flűckiger, Matthias Frank5, Janos Hajdu, Max F. Hantke, Brenda Hogue1, Ahmad Hosseinizadeh6, Mark S. Hunter7, H. Olof Jönsson, Richard Kirian3, Ruslan P. Kurta, Duane Loh, Filipe R. N. C. Maia, Adrian P. Mancuso, Andrew J. Morgan, Matthew McFadden, Kerstin Muehlig, Anna Munke, Hemanth Kumar Narayana Reddy, Carl Nettelblad, Abbas Ourmazd8, Max Rose, Peter Schwander8, M. Marvin Seibert, Jonas A. Sellberg, Raymond G. Sierra, Zhibin Sun, Martin Svenda, Ivan A. Vartanyants, Peter Walter, Daniel Westphal, Garth Williams, P. Lourdu Xavier, Chun Hong Yoon, Sahba Zaare

1. Arizona State University 2. ARC Centre of Excellence for Advanced Molecular Imaging 3. Center for Free-Electron Laser Science 4. Biosciences Division at SLAC National Accelerator Laboratory 5. Lawrence Livermore National Laboratory 6. University of Wisconsin - Milwaukee 7. SLAC National Accelerator Laboratory 8. University of Wisconsin-Milwaukee

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Category

BioXFEL Publications

Published on

Type

journal-article

Author

Haoyuan Li and Reza Nazari and Brian Abbey and Roberto Alvarez and Andrew Aquila and Kartik Ayyer and Anton Barty and Peter Berntsen and Johan Bielecki and Alberto Pietrini and Maximilian Bucher and Gabriella Carini and Henry N. Chapman and Alice Contreras and Benedikt J. Daurer and Hasan DeMirci and Leonie Flűckiger and Matthias Frank and Janos Hajdu and Max F. Hantke and Brenda G. Hogue and Ahmad Hosseinizadeh and Mark S. Hunter and H. Olof Jönsson and Richard A. Kirian and Ruslan P. Kurta and Duane Loh and Filipe R. N. C. Maia and Adrian P. Mancuso and Andrew J. Morgan and Matthew McFadden and Kerstin Muehlig and Anna Munke and Hemanth Kumar Narayana Reddy and Carl Nettelblad and Abbas Ourmazd and Max Rose and Peter Schwander and M. Marvin Seibert and Jonas A. Sellberg and Raymond G. Sierra and Zhibin Sun and Martin Svenda and Ivan A. Vartanyants and Peter Walter and Daniel Westphal and Garth Williams and P. Lourdu Xavier and Chun Hong Yoon and Sahba Zaare

Citation

Li, H. et al., 2020. Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source. Scientific Data, 7(1). Available at: http://dx.doi.org/10.1038/s41597-020-00745-2.

Abstract

AbstractSingle Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

DOI

http://dx.doi.org/10.1038/s41597-020-00745-2

Funding

NSF-STC Biology with X-ray Lasers (NSF-1231306)