Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization
Category
Published on
Type
journal-article
Author
Tek Narsingh Malla and Srinivasan Muniyappan and David Menendez and Favour Ogukwe and Aleksandar N. Dale and Joseph D. Clayton and Dominique D. Weatherall and Prabin Karki and Shishir Dangi and Victoria Mandella and A. Andrew Pacheco and Emina A. Stojković and Samuel L. Rose and Julien Orlans and Shibom Basu and Daniele de Sanctis and Marius Schmidt
Citation
Malla, T. N., Muniyappan, S., Menendez, D., Ogukwe, F., Dale, A. N., Clayton, J. D., Weatherall, D. D., Karki, P., Dangi, S., Mandella, V., Pacheco, A. A., Stojković, E. A., Rose, S. L., Orlans, J., Basu, S., de Sanctis, D., & Schmidt, M. (2025). Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization. IUCrJ, 12(1), 36–48. https://doi.org/10.1107/s2052252524010868
Abstract
The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution. ID29 is a new beamline dedicated to SSX experiments at ESRF–EBS. Here, we report experiments emerging from the initial phase of user operation at ID29. We first used microcrystals of photoactive yellow protein as a model system to exploit the potential of microsecond pulses for SSX. Subsequently, we investigated microcrystals of cytochrome c nitrite reductase (ccNiR) with microsecond X-ray pulses. CcNiR is a decaheme protein that is ideal for the investigation of radiation damage at the various heme-iron sites. Finally, we performed a proof-of-concept subsecond time-resolved SSX experiment by photoactivating microcrystals of a myxobacterial phytochrome.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
