The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina
Category
Published on
Type
journal-article
Author
Abigael J. Kosgei and Mitchell D. Miller and Minakshi Bhardwaj and Weijun Xu and Jon S. Thorson and Steven G. Van Lanen and George N. Phillips
Citation
Kosgei, A.J. et al., 2022. The crystal structure of DynF from the dynemicin-biosynthesis pathway of Micromonospora chersina. Acta Crystallographica Section F Structural Biology Communications, 78(1), pp.1–7. Available at: http://dx.doi.org/10.1107/s2053230x21012322.
Abstract
Dynemicin is an enediyne natural product from Micromonospora chersina ATCC53710. Access to the biosynthetic gene cluster of dynemicin has enabled the in vitro study of gene products within the cluster to decipher their roles in assembling this unique molecule. This paper reports the crystal structure of DynF, the gene product of one of the genes within the biosynthetic gene cluster of dynemicin. DynF is revealed to be a dimeric eight-stranded β-barrel structure with palmitic acid bound within a cavity. The presence of palmitic acid suggests that DynF may be involved in binding the precursor polyene heptaene, which is central to the synthesis of the ten-membered ring of the enediyne core.
DOI
Funding
NSF-STC Biology with X-ray Lasers (NSF-1231306)
