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  1. Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.

    15 Apr 2015 | Publications

    Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for …

  2. Career Trends: Exploring Careers Beyond the Bench

    20 Jan 2015 | Publications

    Last month, the U.S. National Science Foundation (NSF) released a report* with some grim news that confirmed what is painfully obvious to recent Ph.D. graduates in science, technology, engineering, and mathematics (STEM) fields: Unemployment for this cohort is on the rise (at 2.4% in 2010, up …

  3. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.

    05 Dec 2014 | Publications

    Authors: Tenboer, J., Basu, S., Zatsepin, N., Pande, K., Milathianaki, D., Frank, M., Hunter, M., Boutet, S., Williams, G. J., Koglin, J. E., Oberthuer, D., Heymann, M., Kupitz, C., Conrad, C., Coe, J., Roy-Chowdhury, S., Weierstall, U., James, D., Wang, D., Grant, T., Barty, A., Yefanov, O., …

  4. Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.

    11 Sep 2014 | Publications

    Authors: Kupitz, C., Basu, S., Grotjohann, I., Fromme, R., Zatsepin, N. A., Rendek, K. N., Hunter, M. S., Shoeman, R. L., White, T. A., Wang, D., James, D., Yang, J. H., Cobb, D. E., Reeder, B., Sierra, R. G., Liu, H., Barty, A., Aquila, A. L., Deponte, D., Kirian, R. A., Bari, S., Bergkamp, J. J., …

  5. Small Angle X-Ray Scattering as a Complementary Tool for High-Throughput Structural Studies

    21 May 2014 | Publications | Contributor(s): Tom Grant, Edward Snell

    Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained by the ability to form a crystal that diffracts well and NMR is constrained to smaller proteins. …

  6. Phasing coherently illuminated nanocrystals bounded by partial unit cells

    31 Dec 2012 | Publications | Contributor(s): R. Kirian, R. Bean, K. Beyerlein, O. Yevanof, T. White, A. Barty, Henry Chapman

    With the use of highly coherent femtosecond X-ray pulses from a free-electron laser, it is possible to record protein nanocrystal diffraction patterns with far more information than is present in conventional crystallographic diffraction data. It has been suggested that diffraction phases may be …

  7. The Coherent X-ray Imaging Data Bank

    31 Dec 2010 | Publications | Contributor(s): F. R. N. C. Maia The Coherent X-ray Imaging Data Bank (CXIDB) is a database offering scientists access to data from Coherent X-ray Imaging (CXI) experiments, including serial femtosecond crystallography and single particle imaging. The Coherent X-ray Imaging Data Bank Filipe R …