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De novo protein crystal structure determination from X-ray free-electron laser data

08 Jan 2014 | Publications | Contributor(s): G. J. Williams, S. Botha, T. R. Barends, J. E. Koglin, R. L. Shoeman, L. Foucar, I. Schlichting, S. Boutet, R. B. Doak, K. Nass, Mark Messerschmidt

The determination of protein crystal structures is hampered by the need for macroscopic crystals. X-ray free-electron lasers (FELs) provide extremely intense pulses of femtosecond duration, which …

https://www.bioxfel.org/resources/238

Protein structural ensembles are revealed by redefining X-ray electron density noise

06 Jan 2014 | Publications | Contributor(s): J. S. Fraser, P. T. Lang, T. Alber, James Holton

To increase the power of X-ray crystallography to determine not only the structures but also the motions of biomolecules, we developed methods to address two classic crystallographic problems: …

https://www.bioxfel.org/resources/282

Serial femtosecond crystallography of G protein-coupled receptors

19 Dec 2013 | Publications | Contributor(s): S. Boutet, M. Caffrey, D. Wang, R. A. Kirian, T. A. White, C. Wang, I. Grotjohann, K. R. Beyerlein, G. W. Han, S. Basu, A. Barty, V. Katritch, D. Wacker, S. T. Shah, C. Gati, M. M. Seibert, K. N. Rendek, W. Liu, G. J. Williams, R. C. Stevens, N. A. Zatsepin, J. E. Koglin, Henry Chapman, Vadim Cherezov, Petra Fromme, James Holton, Christopher Kupitz, Applications Manager, Mark Messerschmidt, Garrett Charles Nelson, John Spence, Uwe Weierstall

X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and …

https://www.bioxfel.org/resources/290

Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature

25 Apr 2013 | Publications | Contributor(s): N. K. Sauter, A. Miahnahri, W. E. White, J. Messinger, R. W. Grosse-Kunstleve, D. W. Schafer, T. C. Weng, J. Hattne, R. G. Sierra, D. Difiore, M. M. Seibert, P. Glatzel, A. Zouni, J. Yano, S. Boutet, G. J. Williams, V. K. Yach, J. Kern, R. Tran, D. Sokaras, N. Echols, A. Lampe, S. Koroidov, J. E. Koglin, R. Alonso-Mori, C. Glockner, M. J. Latimer, P. D. Adams, A. R. Fry, J. Sellberg, G. Han, U. Bergmann, J. Hellmich, R. J. Gildea, D. Milathianaki, P. H. Zwart, B. Lassalle-Kaiser, M. J. Bogan, S. Gul, H. Laksmono, Mark Messerschmidt, Ranga Setlur

Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of …

https://www.bioxfel.org/resources/293

High-resolution protein structure determination by serial femtosecond crystallography

19 Jul 2012 | Publications | Contributor(s): T. A. White, A. Aquila, D. P. DePonte, L. J. Koerner, R. A. Kirian, G. J. Williams, C. Y. Hampton, S. Boutet, J. Steinbrener, P. A. Montanez, M. Liang, D. Wang, A. V. Martin, J. Defever, N. A. Zatsepin, L. Redecke, R. Herbst, S. M. Gruner, H. T. Philipp, D. Arnlund, M. W. Tate, L. C. Johansson, G. Haller, C. Kenney, J. Pines, R. Neutze, N. van Bakel, M. J. Bogan, T. R. Barends, A. Barty, I. Schlichting, D. Schafer, L. Lomb, R. B. Doak, S. Kassemeyer, G. Katona, K. Nass, P. Hart, M. M. Seibert, F. Stellato, W. Ghonsalves, C. Caleman, J. Morse, R. L. Shoeman, N. Timneanu, M. Hromalik, Henry Chapman, Petra Fromme, Mark S. Hunter, Christopher Kupitz, Mark Messerschmidt, John Spence, Uwe Weierstall

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding …

https://www.bioxfel.org/resources/254

Small-angle X-ray scattering and single-molecule FRET spectroscopy produce highly divergent views of the low-denaturant unfolded state

03 May 2012 | Publications | Contributor(s): J. Hinshaw, T. Y. Yoo, G. Haran, T. R. Sosnick, K. Plaxco, S. P. Meisburger, Lois Pollack

The results of more than a dozen single-molecule Forster resonance energy transfer (smFRET) experiments suggest that chemically unfolded polypeptides invariably collapse from an expanded random coil …

https://www.bioxfel.org/resources/297

In vivo protein crystallization opens new routes in structural biology

29 Feb 2012 | Publications | Contributor(s): A. Barty, N. Kimmel, M. Barthelmess, F. Stellato, T. Ekeberg, C. Bostedt, R. Koopmann, L. Lomb, D. Rehders, S. Stern, J. Hajdu, B. Rudek, R. B. Doak, H. Graafsma, J. Andreasson, A. Rudenko, P. Holl, M. J. Bogan, G. J. Williams, K. Nass, C. Reich, S. Bajt, L. Struder, F. R. Maia, T. A. White, J. Ullrich, C. Betzel, R. Hartmann, H. Soltau, A. Aquila, N. Coppola, L. Gumprecht, D. P. Deponte, S. Kassemeyer, J. Davidsson, B. Erk, R. A. Kirian, M. M. Seibert, H. Fleckenstein, I. Schlichting, A. Hartmann, N. Timneanu, M. Duszenko, G. Hauser, T. Stehle, K. Cupelli, C. Caleman, R. L. Shoeman, L. Redecke, D. Rolles, S. W. Epp, J. Schulz, C. B. Wunderer, R. G. Sierra, H. Hirsemann, J. D. Bozek, A. V. Martin, M. Liang, G. Weidenspointner, X. Wang, L. Foucar, C. Y. Hampton, S. Boutet, Henry Chapman, Petra Fromme, Mark S. Hunter, Mark Messerschmidt, John Spence, Uwe Weierstall

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis.

https://www.bioxfel.org/resources/258

Reconstructing an icosahedral virus from single-particle diffraction experiments

28 Aug 2011 | Publications | Contributor(s): H. C. Poon, CMS Manager, Dilano Saldin, Marius Schmidt, Peter Schwander, Miraj Uddin

The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively …

https://www.bioxfel.org/resources/285

Femtosecond X-ray protein nanocrystallography

02 Feb 2011 | Publications | Contributor(s): G. Schaller, A. Barty, M. Adolph, S. Bajt, D. Rupp, I. Grotjohann, C. Bostedt, T. A. White, J. Krzywinski, D. P. DePonte, N. Timneanu, H. Soltau, B. Erk, M. M. Seibert, S. Stern, L. Gumprecht, G. Hauser, R. Hartmann, T. R. Barends, H. Gorke, A. Rocker, M. Bott, S. Herrmann, K. Nass, F. Krasniqi, R. Andritschke, J. Andreasson, C. D. Schroter, R. Neutze, J. Ullrich, D. Rolles, P. Holl, I. Andersson, B. Rudek, G. Potdevin, A. Aquila, L. Struder, J. Hajdu, C. Y. Hampton, N. Kimmel, X. Wang, H. Hirsemann, A. V. Martin, M. Liang, S. Schorb, L. Foucar, F. Schopper, R. G. Sierra, T. Gorkhover, G. Weidenspointner, A. Homke, M. Barthelmess, S. Boutet, D. Starodub, R. A. Kirian, I. Schlichting, C. Caleman, R. L. Shoeman, J. Schulz, D. Pietschner, O. Jonsson, B. Nilsson, M. Svenda, S. W. Epp, G. J. Williams, L. Lomb, H. Graafsma, A. Rudenko, M. J. Bogan, S. Marchesini, K. U. Kuhnel, F. R. Maia, R. B. Doak, J. D. Bozek, C. Reich, N. Coppola, Henry Chapman, Matthias Frank, Petra Fromme, Stefan Hau-Riege, James Holton, Mark S. Hunter, Mark Messerschmidt, Marius Schmidt, John Spence, Uwe Weierstall

X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the …

https://www.bioxfel.org/resources/252

Single mimivirus particles intercepted and imaged with an X-ray laser

02 Feb 2011 | Publications | Contributor(s): F. R. Maia, A. Aquila, L. Foucar, R. Hartmann, J. Hajdu, D. Odic, N. Coppola, H. Graafsma, B. Nilsson, M. J. Bogan, S. Stern, I. Schlichting, C. D. Schroter, T. Gorkhover, G. Weidenspointner, A. Rudenko, M. Barthelmess, S. Herrmann, D. Starodub, R. Andritschke, G. Hauser, A. Homke, L. Lomb, R. L. Shoeman, J. M. Claverie, D. Pietschner, O. Jonsson, H. Soltau, C. Abergel, M. M. Seibert, R. G. Sierra, J. D. Bozek, C. Y. Hampton, V. Seltzer, H. Gorke, B. Iwan, M. Hantke, S. Schorb, F. Krasniqi, A. A. Miahnahri, K. U. Kuhnel, I. Andersson, C. Caleman, T. Ekeberg, G. Schaller, A. Rocker, M. Adolph, S. Boutet, J. Ullrich, D. Rupp, S. W. Epp, F. Schopper, A. Barty, L. Gumprecht, R. Kirian, J. Krzywinski, D. P. DePonte, N. Kimmel, H. Hirsemann, B. Rudek, M. Liang, R. B. Doak, J. Andreasson, C. Reich, G. Williams, B. Erk, M. Bott, S. Marchesini, D. Westphal, K. O. Hodgson, T. A. White, G. Potdevin, A. Martin, L. Struder, S. Bajt, J. Schulz, D. Rolles, P. Holl, C. Bostedt, M. Svenda, Henry Chapman, Matthias Frank, Petra Fromme, Stefan Hau-Riege, Mark S. Hunter, Mark Messerschmidt, Marius Schmidt, John Spence, Uwe Weierstall

X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray …

https://www.bioxfel.org/resources/294

Reconstruction from a single diffraction pattern of azimuthally projected electron density of molecules aligned parallel to a single axis

31 Dec 2009 | Publications | Contributor(s): V. L. Shneerson, D. Starodub, Dilano Saldin, John Spence

Diffraction from the individual molecules of a molecular beam, aligned parallel to a single axis by a strong electric field or other means, has been proposed as a means of structure determination of …

https://www.bioxfel.org/resources/286