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Extraction of fast changes in the structure of a disordered ensemble of photoexcited biomolecules
Teaching Materials 13 Oct 2015
Using pump-probe experiments of varying time intervals between pump and probe, the method of time-resolved crystallography has given many insights into the fast time variations of crystallized molecules as a result of photoexcitation.… Authors: H.-C. Poon, M. Schmidt, and D. K. Saldin View article Video Summary
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Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Teaching Materials 13 Oct 2015
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser.… This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.……
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Clustering and main sources of variance of SFX data
Teaching Materials 29 Aug 2014
Wolfgang Brehm (University of Konstanz) Clustering approaches and variance analysis are explained using the example of the indexing ambiguity in serial crystallography.… Variance analysis can lead to a more accurate result with less data by correcting the main dependent errors.… Scroll down for video and…
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Rapid Data Quality Feedback and Online Monitoring using CASS
Teaching Materials 29 Aug 2014
Karol Nass (MPI) Description of the layout of CASS software suite and its functionality on the LCLS online and offline data streams.… Rapid offline processing of the raw LCLS data: crystal hit finding, extraction of images into HDF5 or cbf formats, pulse characteristics, fluorescence spectra, other data specific to an…
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Double-focusing mixing jet for XFEL study of chemical kinetics
Teaching Materials 13 Oct 2015
Several liquid sample injection methods have been developed to satisfy the requirements for serial femtosecond X-ray nanocrystallography, which enables radiation-damage-free determination of molecular structure at room temperature.… A windowless liquid mixing jet device has been designed for this purpose.… The…
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Teaching Materials 3 Sep 2014
Chris O'Grady (SLAC) Psana is the main analysis tool developed by LCLS, upon which some other tools are based.… I will discuss recent psana developments and future plans.… reduced size" HDF5 files; ability to run psana on Windows/Mac "laptops" using virtualization. Scroll down for video and interactive…
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Crystallization of the Large Membrane Protein Complex Photosystem I in a Microfluidic Channel
Teaching Materials 9 Oct 2015
Traditional macroscale protein crystallization is accomplished nontrivially by exploring a range of protein concentrations and buffers in solution until a suitable combination is attained. This methodology is time-consuming and resource-intensive, hindering protein structure determination.… Structure determination techniques…
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Deducing fast electron density changes in uncrystallized biomolecules
Teaching Materials 13 Oct 2015
We propose a method for deducing time-resolved structural changes in uncrystallized biomolecules in solution.… The experiment is somewhat like a pump–probe version of an experiment on small angle X-ray scattering, except that the data expected by the algorithm are not just the radial variation of the averaged intensities. The…
