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Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

By Suraj Pandey¹, George Douglas Calvey, Andrea Katz, Tek Narsingh Malla¹, Faisal Hammad Mekky Koua², Jose M. Martin-Garcia, Ishwor Poudyal¹, Jay-How Yang³, Mohammad Vakili, Oleksandr Yefanov, Kara Zielinski⁴, Sasa Bajt, Salah Awel, Katarina Doerner, Matthias Frank⁵, Luca Gelisio, Rebecca Jeanne Jernigan³, Henry Kirkwood, Marco Kloos, Jayanath Koliyadu, Valerio Mariani, Mitchell Miller⁶, Grant Mills, Garrett Charles Nelson³, Jose L. Olmos, Alireza Sadri, Tokushi Sato, Alexandra Tolstikova, Weijun Xu, Abbas Ourmazd⁷, John Spence³, Peter Schwander⁷, Anton Barty, Henry Chapman⁴, Petra Fromme³, Adrian P. Mancuso, George Phillips⁶, Richard Bean, Lois Pollack⁸, Marius Schmidt¹

1. University of Wisconsin - Milwaukee 2. Nagoya Institute of Technology 3. Arizona State University 4. Center for Free-Electron Laser Science 5. Lawrence Livermore National Laboratory 6. Rice University 7. University of Wisconsin-Milwaukee 8. Cornell University