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An Introduction to X-Ray Diffraction Physics
10 Aug 2016 | Teaching Materials | Contributor(s): Blessing
These notes present basic principles of the physics that underlies X-ray analysis of the atomic structure of matter. In particular, classical electromagnetic wave theory of X-ray scattering by …
https://www.bioxfel.org/resources/diffraction-physics
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The Structural History of RNA Polymerase Transcription Machinery
18 Feb 2016 | Teaching Materials | Contributor(s): Roger Kornberg
Dr. Roger Kornberg, 2006 Nobel Laureate in Chemistry, discusses the structural history of the RNA polymerase II transcription machinery and how it played a role in his successful career.
https://www.bioxfel.org/resources/914
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Interview with Dilano Saldin
05 Jan 2016 | Teaching Materials
Combining data from new facilities with mathematical methods, Professor Dilano Saldin is in pursuit of a technique to determine the structure of molecules without the need for purification …
https://www.bioxfel.org/resources/868
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Multitemperature data and diffuse scattering in X-ray crystallography
07 Dec 2015 | Teaching Materials | Contributor(s): James Fraser
Dr. James Fraser will discuss recent advances in mapping the structural basis of protein conformational dynamics using multitemperature data and diffuse scattering from both conventional synchrotron …
https://www.bioxfel.org/resources/865
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BioXFEL Graduate Student Cross-Training Scholarship
11 Nov 2015 | Teaching Materials
BioXFEL is committed to providing our students with opportunities to diversify their skill sets and integrate themselves into our highly multidisciplinary Center. In addition to the workshops, …
https://www.bioxfel.org/resources/CTS
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BioXFEL Scholar Application Form
10 Nov 2015 | Teaching Materials
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https://www.bioxfel.org/resources/658
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Serial femtosecond crystallography of soluble proteins in lipidic cubic phase
10 Nov 2015 | Teaching Materials
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal …
https://www.bioxfel.org/resources/681
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Indexing ambiguity in SFX resolved using expectation maximization algorithm
10 Nov 2015 | Teaching Materials
Crystallographic auto-indexing algorithms provide crystal orientations and unit-cell parameters and assign Miller indices based on the geometric relations between the Bragg peaks observed in …
https://www.bioxfel.org/resources/680
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Introduction to XRayView Crystallographic Software
04 Nov 2015 | Teaching Materials
Dr. George Phillips introduces the basic concepts of crystallography focusing on the reciprocal lattice and Ewald sphere construction and using the XRayView software developed in his lab to help …
https://www.bioxfel.org/resources/679
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Overview of Serial Femtosecond Crystallography
14 Oct 2015 | Teaching Materials | Contributor(s): Petra Fromme
Dr. Petra Fromme discusses an introductory overview to the field of serial crystallography and recent results showing the potential power of the technique.
https://www.bioxfel.org/resources/668
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Virus Crystallography
14 Oct 2015 | Teaching Materials | Contributor(s): Robert M Lawrence
Dr. Rob Lawrence describes the unique problems associated with crystallization of virus particles and different methods to overcome them.
https://www.bioxfel.org/resources/667
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When Your Crystals Bite
14 Oct 2015 | Teaching Materials | Contributor(s): George Phillips
Dr. George Phillips describes properties of crystals such as twinning and other defects and how they can adversely affect crystallographic analysis.
https://www.bioxfel.org/resources/666
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Optimizing crystallization hits - small to large and large to small
14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft
Joe Luft describes methods for optimizing initial results from crystallization screening trials for both macro and nanocrystals.
https://www.bioxfel.org/resources/665
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Practical Considerations for Crystallographic Data Analysis
14 Oct 2015 | Teaching Materials | Contributor(s): Tom Grant
Dr. Thomas Grant explains how crystal quality affects diffraction data and how you can detect and ameliorate problems in crystallographic data.
https://www.bioxfel.org/resources/664
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Applications of TEM for Evaluation and Optimization of Crystal Growth
14 Oct 2015 | Teaching Materials | Contributor(s): Guillermo Calero
Dr. Guillero Calero discusses using transmission electron microscopy to characterize, evaluate and subsequently optimize the growth of crystals.
https://www.bioxfel.org/resources/663
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What is the outcome of your crystallization experiment
14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft
Joe Luft discusses the detection of hits in crystallization screening experiments for both macro and nanocrystals.
https://www.bioxfel.org/resources/662
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Strategies to sample crystallization space
14 Oct 2015 | Teaching Materials | Contributor(s): Joseph Luft
Joe Luft discusses crystallization screening strategies to maximize the likelihood of success.
https://www.bioxfel.org/resources/661
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Fundamentals of Crystal Growth
14 Oct 2015 | Teaching Materials | Contributor(s): Edward Snell, Eaton E Lattman
Dr. Ed Lattman discusses the foundations of crystallography and theoretical and practical considerations including X-ray radiation and production and crystallization nucleation, growth and …
https://www.bioxfel.org/resources/660
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Using XFELs to measure the solution structures of biomolecules
13 Oct 2015 | Teaching Materials
Dr. Lois Pollack presents an introduction to small and wide angle X-ray solution scattering techniques and describes the advances made possible by XFELs.
https://www.bioxfel.org/resources/657
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Single Particle Reconstruction
13 Oct 2015 | Teaching Materials
Dr. Dilano Saldin discusses recent advances in algorithms for single particle reconstruction from solution scattering data at XFELs.
https://www.bioxfel.org/resources/656
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Extraction of fast changes in the structure of a disordered ensemble of photoexcited biomolecules
13 Oct 2015 | Teaching Materials
Using pump-probe experiments of varying time intervals between pump and probe, the method of time-resolved crystallography has given many insights into the fast time variations of crystallized …
https://www.bioxfel.org/resources/655
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Ab-initio phasing using nanocrystal shape transforms with incomplete unit cells
13 Oct 2015 | Teaching Materials
X-ray free electron lasers are used in measuring diffraction patterns from nanocrystals in the `diffract-before-destroy' mode by outrunning radiation damage. The finite-sized nanocrystals provide an …
https://www.bioxfel.org/resources/654
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Post-refinement method for snapshot serial crystallography
13 Oct 2015 | Teaching Materials
A post-refinement procedure has been devised for ‘snapshot’ diffraction data consisting entirely of partially recorded reflections, each diffraction pattern from a crystal in an orientation …
https://www.bioxfel.org/resources/653
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Mapping the continuous reciprocal space intensity of X-ray serial crystallography
13 Oct 2015 | Teaching Materials
Serial crystallography using X-ray free-electron lasers enables the collection of tens of thousands of measurements from an equal number of individual crystals, each of which can be smaller than 1 …
https://www.bioxfel.org/resources/652
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Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
13 Oct 2015 | Teaching Materials
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton …
https://www.bioxfel.org/resources/651
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Double-focusing mixing jet for XFEL study of chemical kinetics
13 Oct 2015 | Teaching Materials
Several liquid sample injection methods have been developed to satisfy the requirements for serial femtosecond X-ray nanocrystallography, which enables radiation-damage-free determination of …
https://www.bioxfel.org/resources/650
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High-resolution structure of viruses from random diffraction snapshots
13 Oct 2015 | Teaching Materials
The advent of the X-ray free-electron laser (XFEL) has made it possible to record diffraction snapshots of biological entities injected into the X-ray beam before the onset of radiation damage. …
https://www.bioxfel.org/resources/649
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Deducing fast electron density changes in uncrystallized biomolecules
13 Oct 2015 | Teaching Materials
We propose a method for deducing time-resolved structural changes in uncrystallized biomolecules in solution. The method relies on measuring the angular correlations of the intensities, when averaged …
https://www.bioxfel.org/resources/648
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Serial femtosecond crystallography of G-protein coupled receptors
13 Oct 2015 | Teaching Materials
G protein–coupled receptors (GPCRs) are eukaryotic membrane proteins that have a central role in cellular communication and have become key drug targets. To overcome the difficulties of growing …
https://www.bioxfel.org/resources/647
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Crystallization of the Large Membrane Protein Complex Photosystem I in a Microfluidic Channel
09 Oct 2015 | Teaching Materials
Traditional macroscale protein crystallization is accomplished nontrivially by exploring a range of protein concentrations and buffers in solution until a suitable combination is attained. This …
https://www.bioxfel.org/resources/646
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3D Single Particle Structure Recovery
07 Oct 2015 | Teaching Materials | Contributor(s): Ahmad Hosseinizadeh
Dr. Ahmad Hosseinizadeh discuss different pathways to recovering the structure of single particles from XFEL snapshots.
https://www.bioxfel.org/resources/645
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Energy Landscapes
07 Oct 2015 | Teaching Materials | Contributor(s): Ali Dashti
Energy landscapes and continuous conformational mapping of molecular machines.
https://www.bioxfel.org/resources/644
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Manifold Embedding
07 Oct 2015 | Teaching Materials | Contributor(s): Peter Schwander
From single particle snapshots to movies of molecular machines and energy landscapes.
https://www.bioxfel.org/resources/643
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Angular Momentum Decomposition
07 Oct 2015 | Teaching Materials | Contributor(s): Dilano Saldin
Dr. Dilano Saldin discusses using angular momentum decomposition in the analysis of single and multi-particle imaging with XFELs.
https://www.bioxfel.org/resources/642