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Results (page 2 of 4)
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Members
Nadia Zatsepin was an Assistant Professor (Research) at Arizona State University and Head of XFEL data analysis in BioXFEL from 2013 to 2019, when she moved to La Trobe University, Australia. Her research focus is on the application and development of serial femtosecond crystallography (SFX) at X-ray free electron lasers (XFELs), and serial…
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BioXFEL Journal Club - Ganesh Subramanian
Events 18 Feb 2015
Feb 18 2015
Ganesh Subramanian will present the following article: Kupitz, C., Basu, S., et al. (2014). Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.
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First Experiments in Structural Biology at the European X-ray Free-Electron Laser
BioXFEL Publications 25 May 2020
http://dx.doi.org/10.3390/app10103642 Ultrabright pulses produced in X-ray free-electron lasers (XFELs) offer new possibilities for industry and research, particularly for biochemistry and pharmaceuticals. The unprecedented brilliance of these next-generation sources enables structure determination from sub-micron crystals as well as…
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BioXFEL Publications 31 Jan 2024 Contributor(s): Marius Schmidt, Emina Stojkovic
http://dx.doi.org/10.1063/4.0000233 Time-resolved crystallography (TRX) is a method designed to investigate functional motions of biological macromolecules on all time scales.… TR serial crystallography (TR-SX) is an extension of TRLX. As the foundations of TRLX were evolving from the late 1980s to the turn of the millennium,…
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BioXFEL Publications 31 Jan 2024 Contributor(s): Marius Schmidt, Emina Stojkovic
https://doi.org/10.1063/4.0000233 Time-resolved crystallography (TRX) is a method designed to investigate functional motions of biological macromolecules on all time scales.… TR serial crystallography (TR-SX) is an extension of TRLX. As the foundations of TRLX were evolving from the late 1980s to the turn of the millennium, TR-SX…
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Puerto Rico Assistance Programs
Education 29 Sep 2017
On September 20th, Hurricane Maria made landfall over Puerto Rico and devastated the island.… It will likely take months to restore a sense of normalcy and years to fully recover. Many of the laboratories have lost all of their samples during the subsequent power outages and are now contaminated with mold. It will take…
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BioXFEL Publications 24 May 2017 Contributor(s): Chelsie E Conrad, Garrett Charles Nelson, Natasha Stander, Nadia Zatsepin, James D Zook, Lan Zhu, Nirupa Nagaratnam, Shatabdi Roy-Chowdhury, Jesse David Coe, GANESH SUBRAMANIAN, Uwe Weierstall, John Spence, Vadim Cherezov, Petra Fromme, Wei Liu
http://dx.doi.org/10.1107/s205225251700570x Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources.… Owing to the longer exposure times that are needed at synchrotrons, serial…
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Observations of phase changes in monoolein during high viscous injection
BioXFEL Publications 21 Mar 2022 Contributor(s): Mark S. Hunter, Richard Kirian, Derek Mendez, Max Oliver Wiedorn, Brian Abbey
http://dx.doi.org/10.1107/s1600577522001862 Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam.… However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not…
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BioXFEL Journal Club - Bobby Abdallah
Events 18 Feb 2015
Feb 18 2015
Bobby Abdallah will present the following article: Tenbauer, J., Basu, S., et. al. (2014). Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.
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Groups
Research in Dr. Fraser’s lab focuses on how protein conformational ensembles respond to perturbations such as temperature, ligand binding, and mutation. The lab develops new data collection capabilities (with a focus on ambient temperature data collection and diffuse scattering) and creates new multiconfomer representations of protein structure…
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Researchers capture membrane protein folding for first time in 3D and at single-atom level
News 3 Jan 2017
Membrane proteins are popular drug targets, as they are exposed to the environment surrounding the cell. Capturing their movements in video, the authors say, is potentially a revolutionary step forward in drug development.… Lead author Eriko Nango of Kyoto University explains that, whereas conventional X-ray crystallography only…
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Legal 2 May 2012
You are responsible at all times for using in a manner that is legal, ethical, and not to the detriment of others.… All members are encouraged to help moderate content and click on "… links to report any material deemed offensive or inappropriate.… If the material is judged to be…
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Time-resolved crystallography and protein design
Publications 1 Jan 2013
https://www.bioxfel.org/resources/Time-resolved-crystallography-and-protein-design
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FD 171: Approaches to time-resolved diffraction using an XFEL
Publications 1 Jan 2011 Contributor(s): John Spence
https://www.bioxfel.org/resources/FD-171:-Approaches-to-time-resolved
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Members
Deanna Clark pursued studies in communications and journalism at the University of Minnesota, where she received a Bachelor of Arts degree.… A proud recipient of the Physics Department's, Outstanding Staff Award (2013) and three-time winner of the Sun Award, Ms.… She has been on staff at ASU since 2009. Ms.…
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Extraction of Fast Changes in the Structure of a Disordered Ensemble of Photoexcited Biomolecules
BioXFEL Publications 22 May 2013
journal-articleH.-C. Poon and M. Schmidt and D. K. SaldinPoon, H.-C., Schmidt, M. & Saldin, D.K., 2013. Extraction of Fast Changes in the Structure of a Disordered Ensemble of Photoexcited Biomolecules.… 1–5. Available at: http://dx.doi.org/10.1155/2013/750371.Using pump-probe experiments of varying time intervals between pump…
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BioXFEL Publications 2 May 2013 Contributor(s): Vadim Cherezov
http://dx.doi.org/10.1107/s0909049513007942 Nonlinear optical (NLO) instrumentation has been integrated with synchrotron X-ray diffraction (XRD) for combined single-platform analysis, initially targeting applications for automated crystal centering. Second-harmonic-generation microscopy and two-photon-excited ultraviolet fluorescence microscopy…
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Coherent Convergent-Beam Time-resolved X-ray Diffraction
Publications 1 Jan 2011 Contributor(s): John Spence, Applications Manager
https://www.bioxfel.org/resources/Coherent-Convergent-Beam-Time-resolved-X-ray-Diffraction
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Real-time observation of the initiation of RNA polymerase II transcription
BioXFEL Publications 2 Sep 2015 Contributor(s): Roger Kornberg
journal-article http://dx.doi.org/10.1038/nature14882 Fazal, F.M. et al., 2015. Real-time observation of the initiation of RNA polymerase II transcription.
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Serial millisecond crystallography of membrane
News 6 Jun 2017
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources.… Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial…
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Events 18 Mar 2015
Mar 18 2015
LG09 - Macromolecular Dynamics with Femtosecond Time Resolution
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Biography 21 May 2013
Marius Schmidt Associate Professor University of Wisconsin- Milwaukee Physics Department Room 414 1900 East Kenwood Boulevard Milwaukee, WI 53211 smarius@uwm.… Marius Schmidt is using physical methods to investigate biological molecules. He is concentrating mainly on static and time-resolved macromolecular crystallography to…
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Time-Resolved Spectra from Millivolt EELS Data
BioXFEL Publications 30 May 2014 Contributor(s): Chufeng Li, GANESH SUBRAMANIAN, John Spence
http://dx.doi.org/10.1017/s1431927614000890 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Li, C., Subramanian, G. & Spence, J.C.H., 2014. Time-Resolved Spectra from Millivolt EELS Data.
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Members
My name is Gabriela Casanova Sepulveda. I’m a junior in the University of Puerto Rico- Mayaguez Campus and my major is Industrial Biotechnology. I am a two-time BioXFEL undergrad summer research student.… Also my interest in structural biology and biophysics was discovered through these experiences. I aspire to enter graduate…
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Structural biology in the time of COVID-19: perspectives on methods and milestones
BioXFEL Publications 22 Apr 2021 Contributor(s): Miranda Lynch, Edward Snell, Sarah EJ Bowman
http://dx.doi.org/10.1107/s2052252521003948 The global COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has wreaked unprecedented havoc on global society, in terms of a huge loss of life and burden of morbidity, economic upheaval and social disruption.… Structural biology has been at the…
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Time-resolved cryogenic electron tomography for the study of transient cellular processes
BioXFEL Publications 8 May 2024 Contributor(s): Kara Zielinski, Christopher Kupitz, Mark S. Hunter, Lois Pollack
https://doi.org/10.1091/mbc.e24-01-0042 Cryogenic electron tomography (cryo-ET) is the highest resolution imaging technique applicable to the life sciences, enabling subnanometer visualization of specimens preserved in their near native states. The rapid plunge freezing process used to prepare samples lends itself to time-resolved studies, which…
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Publications 1 Jan 2013
https://www.bioxfel.org/resources/Singular-Value-Decomposition-as-a
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Time-resolved cryogenic electron tomography for the study of transient cellular processes
BioXFEL Publications 8 May 2024 Contributor(s): Kara Zielinski, Christopher Kupitz, Mark S. Hunter, Lois Pollack
http://dx.doi.org/10.1091/mbc.e24-01-0042 Cryogenic electron tomography (cryo-ET) is the highest resolution imaging technique applicable to the life sciences, enabling subnanometer visualization of specimens preserved in their near native states. The rapid plunge freezing process used to prepare samples lends itself to time-resolved studies, which…
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Events 9 Dec 2015
Dec 09 2015
LK17 - Time-Resolved Serial Femtosecond Nanocrystallography to Establish Structure-Based Enzymology at the LCLS
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Publications 1 Jan 2010 Contributor(s): Marius Schmidt, Dilano Saldin
https://www.bioxfel.org/resources/Enzyme-transient-state-kinetics-in
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BioXFEL Scientist, Dilano Saldin, passes away
News 24 Mar 2020
BioXFEL scientist Dilano Saldin, Distinguished Professor of Physics of the University of Wisconsin-Milwaukee and Fellow of the American Physical Society, passed away on March 9, 2020 after a long illness. He will be greatly missed by his family, friends, and the scientific community. Below is his obituary. Remembering Dilano Saldin For four…
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Towards a generalised approach for the time-resolved crystallographic study of enzymes
BioXFEL Publications 5 Jan 2016 Contributor(s): Edward Snell
http://dx.doi.org/10.1107/s205327331509957x Diana C. F. Monteiro and Vijay Patel and Christopher P. Bartlett and Shingo Nozaki and Thomas D. Grant and James A. Gowdy and Gary S. Thompson and Arnout P. Kalverda and Edward H. Snell and Hironori Niki and Arwen R. Pearson and Michael E. Webb Monteiro, D.C.F. et al., 2015. Towards a generalised approach…
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Time-Resolved Contrast Variation SAXS for Studying RNA-Protein Interactions
BioXFEL Publications 15 Feb 2019 Contributor(s): Andrea Katz, George Douglas Calvey, Lois Pollack
http://dx.doi.org/10.1016/j.bpj.2018.11.1733 poster Pabit, S.A. et al., 2019. Time-Resolved Contrast Variation SAXS for Studying RNA-Protein Interactions.
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Time-resolved mixing-jet X-ray free-electron laser crystallography experiments
BioXFEL Publications 19 Dec 2017 Contributor(s): Max Oliver Wiedorn, Henry Chapman
http://dx.doi.org/10.1107/s2053273317087393 journal-article Oberthuer, D. et al., 2017. Time-resolved mixing-jet X-ray free-electron laser crystallography experiments.
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Publications 1 Jan 2011
https://www.bioxfel.org/resources/Opportunities-and-challenges-for-time-resolved
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Photosynthesis seen in a new light by rapid X-ray pulses
News 12 Nov 2019
In a new study led by Petra Fromme and Nadia Zatsepin at the Biodesign Center for Applied Structural Discovery, the School of Molecular Sciences and the Department of Physics at Arizona State University, researchers investigated the structure of Photosystem I (PSI) with ultrashort X-ray pulses at the European X-ray Free Electron Laser (EuXFEL)…
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Applications of microfluidic mixers for time-resolved SAXS and crystallography experiments
BioXFEL Publications 11 May 2021 Contributor(s): Kara Zielinski, Lois Pollack
http://dx.doi.org/10.1107/s0108767320099663 journal-article Zielinski, K. & Pollack, L., 2020. Applications of microfluidic mixers for time-resolved SAXS and crystallography experiments.
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Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources
BioXFEL Publications 1 Jun 2017 Contributor(s): Marius Schmidt
http://dx.doi.org/10.1007/978-1-4939-7000-1_11 book-chapter NSF-STC Biology with X-ray Lasers (NSF-1231306) Schmidt, M., 2017. Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources.
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Wiki 22 May 2014
The Wiki supports !LaTeX markup:pifrac sqrt+24 int_0^dxMathematical Formula (!LaTeX) can be inserted into text like this:Insert formula hereFor example:...displays alpha^2+beta^21 Displaying a Formula The Wiki uses a subset of !TeX markup, including some extensions from !LaTeX and !AMSLaTeX, for mathematical formulae. It generates either PNG images…
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Events 3 Dec 2021
Dec 03 2021
8th Call for Regular Proposals for User Experiments, call for Protein Crystal Screening Proposals and Expressions of Interest in Long-Term Proposals The 8th call for regular proposals at European X-Ray Free-Electron Laser Facility (European XFEL) is open until Friday 03 December 2021 16:00 (local Hamburg/Schenefeld time – Central European Time…
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Coherent convergent-beam time-resolved X-ray diffraction
BioXFEL Publications 10 Jun 2014
http://dx.doi.org/10.1098/rstb.2013.0325 J. C. H. Spence and N. A. Zatsepin and C. Li Spence, J.C.H., Zatsepin, N.A. & Li, C., 2014. Coherent convergent-beam time-resolved X-ray diffraction.
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BioXFEL Publications 11 Apr 2015
http://dx.doi.org/10.1063/1.4916980 K. Pande and M.… Schwander and D.… Saldin Pande, K.… , 2015. Simulations on time-resolved structure determination of uncrystallized biomolecules in the presence of shot noise.
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SLAC's Electron Hub Gets New 'Metro Map' for World's Most Powerful X-Ray Laser
News 7 Jul 2017
The central hub for powerful electron beams at the Department of Energy’s SLAC National Accelerator Laboratory is getting a makeover to prepare for the installation of LCLS-II – a major upgrade to the Linac Coherent Light Source (LCLS), the world’s first hard X-ray free-electron laser.… The hub, called the Beam Switch…
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BioXFEL Publications 18 Apr 2015 Contributor(s): Jesse David Coe, Christopher Kupitz, Shibom Basu, Chelsie E Conrad, Shatabdi Roy-Chowdhury, Petra Fromme
http://dx.doi.org/10.1016/bs.mie.2015.01.011 Jesse Coe and Christopher Kupitz and Shibom Basu and Chelsie E. Conrad and Shatabdi Roy-Chowdhury and Raimund Fromme and Petra Fromme Coe, J. et al., 2015. Crystallization of Photosystem II for Time-Resolved Structural Studies Using an X-ray Free Electron Laser.
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Groups
Dr. Frank's lab is currently working on developing and demonstrating techniques for high-resolution, dynamical imaging of biomolecules and biological nanoparticles with X-ray free-electron lasers, such as the Linac Coherent Light Source at SLAC.… As part of the BioXFEL Center, Dr. Frank, is a leader in the…
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Transient state measurements on proteins by time-resolved crystallography
BioXFEL Publications 22 Apr 2022 Contributor(s): Tek Narsingh Malla, Marius Schmidt
http://dx.doi.org/10.1016/j.sbi.2022.102376 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Malla, T. N., & Schmidt, M. (2022). Transient state measurements on proteins by time-resolved crystallography.
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Virus Dynamics Studied by Time-Resolved Small Angle X-Ray Scattering
BioXFEL Publications 15 Feb 2019 Contributor(s): Lois Pollack
http://dx.doi.org/10.1016/j.bpj.2018.11.2321 journal-article San Emeterio, J., & Pollack, L. (2019). Virus Dynamics Studied by Time-Resolved Small Angle X-Ray Scattering.
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Advances in microfluidic mixers for time-resolved structural biology with X-rays
BioXFEL Publications 23 May 2025 Contributor(s): Kara Zielinski, Lois Pollack
https://doi.org/10.1007/s12551-025-01321-x journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Zielinski, K. A., & Pollack, L. (2025). Advances in microfluidic mixers for time-resolved structural biology with X-rays.
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BioXFEL Publications 28 Mar 2014 Contributor(s): Marius Schmidt, Dilano Saldin
http://dx.doi.org/10.1063/1.4869472 Marius Schmidt and Dilano K. Saldin Schmidt, M. & Saldin, D.K., 2014. Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer.
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Events 11 Dec 2019
Dec 11 2019
Call for Proposals User Experiments at the European XFEL Deadline: 11 December 2019 16:00 - Hamburg/Schenefeld time – CET … (Note the unusual deadline time!… The European X-Ray Free-Electron Laser Facility (European XFEL, https://www.xfel.eu) encourages the scientific community worldwide to submit proposals…
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Groups
The John Spence Group will focus on development of new single-particle sample-delivery systems for viruses and macromolecules, which aim to improve the hit rate from its current value of about 1% in gas-phase injectors, which are free of water background.… These include use expectation maximization for partial reflections and…
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BioXFEL Publications 29 Aug 2016 Contributor(s): George Douglas Calvey, Andrea Katz, Lois Pollack
http://dx.doi.org/10.1063/1.4961971 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Calvey, G.D. et al., 2016. Mixing injector enables time-resolved crystallography with high hit rate at X-ray free electron lasers.
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Difference structures from time-resolved small-angle and wide-angle x-ray scattering
BioXFEL Publications 17 May 2018 Contributor(s): Prakash Nepal
http://dx.doi.org/10.1103/physrevb.97.195426 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Nepal, P. & Saldin, D.K., 2018. Difference structures from time-resolved small-angle and wide-angle x-ray scattering.
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9th Annual International Conference
Published 20 Apr 2021
No details available at this time.
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News 6 Apr 2017
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain.… Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus…
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Watching proteins function with time-resolved x-ray crystallography
BioXFEL Publications 3 Jul 2017 Contributor(s): Marius Schmidt
http://dx.doi.org/10.1088/1361-6463/aa7d32 journal-article Šrajer, V. & Schmidt, M., 2017. Watching proteins function with time-resolved x-ray crystallography.
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Functional Expansion of G-Protein-Coupled Receptor Shown by Time-Resolved X-Ray Scattering
BioXFEL Publications 12 Feb 2021 Contributor(s): Konstantinos Karpos, Petra Fromme, Richard Kirian
http://dx.doi.org/10.1016/j.bpj.2020.11.1003 journal-article Menon, C.S.K. et al., 2021. Functional Expansion of G-Protein-Coupled Receptor Shown by Time-Resolved X-Ray Scattering.
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Members
Hasan DeMirci is a member of the Biosciences Division at SLAC National Accelerator Laboratory and also affiliated with Non-Periodic Imaging group at Stanford PULSE Institute.… S. at Bosphorus University in 2002 and later obtained a Ph.D., in Molecular Biology, Cell Biology and Biochemistry at Brown University in 2007. His research…
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Following Folding Pathways of Common Riboswitch Motifs with Time-Resolved Single-Molecule FRET
BioXFEL Publications 15 Feb 2019 Contributor(s): Lois Pollack
http://dx.doi.org/10.1016/j.bpj.2018.11.1931 poster Plumridge, A. & Pollack, L., 2019. Following Folding Pathways of Common Riboswitch Motifs with Time-Resolved Single-Molecule FRET.
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BioXFEL Publications 12 Jun 2015 Contributor(s): Chufeng Li, John Spence
journal-articleChufeng Li and Kevin Schmidt and John C. SpenceLi, C., Schmidt, K. & Spence, J.C., 2015. Data collection strategies for time-resolved X-ray free-electron laser
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BioXFEL Publications 7 Mar 2018 Contributor(s): GANESH SUBRAMANIAN, Uwe Weierstall, John Spence
http://dx.doi.org/10.1021/acs.jpclett.8b00083 journal-article NSF-STC Biology with X-ray Lasers (NSF-1231306) Subramanian, G. et al., 2018. Direct Structural and Chemical Characterization of the Photolytic Intermediates of Methylcobalamin Using Time-Resolved X-ray Absorption Spectroscopy.… 1542–1546. Available at:…
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Time-resolved structural studies with serial crystallography: A new light on retinal proteins
BioXFEL Publications 29 Jun 2015 Contributor(s): Gebhard F.X. Schertler
journal-article http://dx.doi.org/10.1063/1.4922774 NSF-STC Biology with X-ray Lasers (NSF-1231306) Panneels, V. et al., 2015. Time-resolved structural studies with serial crystallography: A new light on retinal proteins.… 041718. Available at: http://dx.doi.org/10.1063/1.4922774. Valérie Panneels and Wenting Wu and Ching-Ju Tsai…
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Time-Resolved Crystallography at X-ray Free Electron Lasers and Synchrotron Light Sources
BioXFEL Publications 9 Dec 2015 Contributor(s): Marius Schmidt
http://dx.doi.org/10.1080/08940886.2015.1101324 Marius Schmidt Schmidt, M., 2015. Time-Resolved Crystallography at X-ray Free Electron Lasers and Synchrotron Light Sources.
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BioXFEL Publications 17 Mar 2023
http://dx.doi.org/10.1107/s2053273322095742 journal-article Casadei, C.… , Schertler, G., Ourmazd, A., & Santra, R. (2022). Randomness in time-resolved serial crystallography data: alternatives to the binning and averaging approach.… https://doi.org/10.1107/s2053273322095742 C. Casadei and A.
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BioXFEL Publications 14 Oct 2022 Contributor(s): Marius Schmidt
http://dx.doi.org/10.1016/j.sbi.2022.102481 journal-article Westenhoff, S., Meszaros, P., & Schmidt, M. (2022). Protein motions visualized by femtosecond time-resolved crystallography: The case of photosensory vs photosynthetic proteins.
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Science 11 Sep 2013
X-ray Free Electron Lasers (XFEL) produce a stream of unimaginably intense X-rays and in unimaginably short pulses, so intense and so rapid that they can capture the fastest biological processes. X-ray Free Electron Lasers offer a unique and exciting opportunity to explore biological mechanisms in areas that were previously thought…
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Towards time-resolved serial crystallography in a microfluidic device
BioXFEL Publications 26 Jun 2015 Contributor(s): Marius Schmidt, Sarah Perry
Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single-crystal strategies. The use of serial data-collection strategies can circumvent challenges associated with radiation damage and repeated reaction initiation. This work utilizes a microfluidic…
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Time-resolved cryogenic electron tomography using mix-and-inject microfluidic devices
BioXFEL Publications 10 Apr 2025 Contributor(s): Kara Zielinski, Christopher Kupitz, Mark S. Hunter, Lois Pollack
https://doi.org/10.1063/4.0000362 Cryogenic electron tomography (cryo-ET) is the highest resolution three-dimensional imaging technique applicable to the life sciences, enabling sub-nanometer visualization of rapidly frozen biological specimens preserved in their near native states. The rapid freezing process lends itself to time-resolved studies,…
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BioXFEL Members Explore Antibiotic Inactivation by a β-Lactamase
News 10 Sep 2018
Antibiotic resistance is an imminent threat to human health. Tuberculosis is the number one killer among infectious diseases. Bacteria that cause tuberculosis are difficult to treat because they are often antibiotic resistant. One of the causes of antibiotic resistance are β-lactamases which broadly inactivate β-lactam antibiotics such as…
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Listen in on X-ray laser science
News 17 Aug 2018
The European X-ray Free-Electron Laser recently came on-line as the biggest and brightest source of X-rays on planet Earth. Those brilliant X-ray beams will allow chemists to do groundbreaking research on enzymes, solar-cell materials, and more. But with great science comes great responsibility. In our latest Stereo Chemistry podcast, C&EN…
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BioXFEL Publications 28 Nov 2017 Contributor(s): Marius Schmidt, Dilano Saldin
http://dx.doi.org/10.1063/1.5016802 journal-article Schmidt, M. & Saldin, D.K., 2017. Publisher’s Note: “Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer” [Struct.
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BioXFEL Publications 16 Sep 2013 Contributor(s): Marius Schmidt
http://dx.doi.org/10.3390/ijms140918881 journal-article Purwar, N. et al., 2013. Spectroscopic Studies of Model Photo-Receptors: Validation of a Nanosecond Time-Resolved Micro-Spectrophotometer Design Using Photoactive Yellow Protein and α-Phycoerythrocyanin.
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BioXFEL Researcher Alexandra Ros Receives Innovation Award
News 2 Nov 2018
BioXFEL researcher, Alexandra Ros of Arizona State University, has received the Federation of Analytical Chemistry and Spectroscopy Societies Innovation Award for her paper and presentation, “Electrically Triggered Water-in-Oil Droplets for Serial Femtosecond Crystallography.… The award is given to the most innovative research…
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BioXFEL Postdoctoral Fellowship Award
News 1 Jul 2021
Sabine Botha is a Postdoctoral Research Scholar in the group of Prof. John Spence at Arizona State University. She completed her undergraduate education and masters program at the University of Oxford (UK) in Engineering Science, specializing in Biomedical and Information Engineering.… After spending 2 years on sample injection…
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BioXFEL Publications 15 Sep 2022 Contributor(s): Kara Zielinski, Andrea Katz, George Douglas Calvey, Lois Pollack
http://dx.doi.org/10.1107/s2053273322099715 journal-article Zielinski, K., Katz, A., Calvey, G., Pabit, S., San Emeterio, J., & Pollack, L. (2022). Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering.
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Diamond Light Source Seeks Beamline Scientist
Opportunities 14 Sep 2021
The XFEL Hub at Diamond Light Source is seeking a Beamline Scientist for a full time, permanent position.… To this end, the Hub is developing tools and methods to enable time-resolved serial crystallography experiments conducted at near-physiological temperatures, especially at XFELs but also at Diamond MX…
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X-ray scattering exhibits time-resolved view of rhodopsin activation
BioXFEL Publications 10 Feb 2023 Contributor(s): Konstantinos Karpos, Petra Fromme, Richard Kirian
http://dx.doi.org/10.1016/j.bpj.2022.11.1170 journal-article Menon, C. S. K., Karpos, K., Grant, T. D., Struts, A. V., Fried, S. D. E., Perera, S. M. D. C., Kosheleva, I. V., Salas-Estrada, L., Grossfield, A., Fromme, P., Kirian, R. A., & Brown, M. F. (2023). X-ray scattering exhibits time-resolved view of rhodopsin activation.
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Protein energy landscapes determined by five-dimensional crystallography
BioXFEL Publications 19 Nov 2013 Contributor(s): Marius Schmidt
Free-energy landscapes decisively determine the progress of enzymatically catalyzed reactions [Cornish-Bowden (2012),Fundamentals of Enzyme Kinetics, 4th ed.… Time-resolved macromolecular crystallography unifies transient-state kinetics with structure determination [Moffat (2001),Chem.… 101, 1569–1581; Schmidtet…
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Time-resolved functional expansion of visual rhodopsin detected by x-ray scattering
BioXFEL Publications 11 Feb 2022 Contributor(s): Konstantinos Karpos, Petra Fromme, Richard Kirian
http://dx.doi.org/10.1016/j.bpj.2021.11.1313 journal-article Menon, C. S. K., Karpos, K., Grant, T. D., Struts, A. V., Fried, S. D. E., Alvarez, R. C., Perera, S. M. D. C., Kosheleva, I. V., Grossfield, A., Fromme, P., Kirian, R. A., & Brown, M. F. (2022). Time-resolved functional expansion of visual rhodopsin detected by x-ray scattering.
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Members
Dr. Messerschmidt studied chemistry and received his PhD from the crystallography department in 2004 at the Free University Berlin, Germany.… He then joined DESY, Hamburg as a research scientist to participating in experimental design and early commissioning of the LCLS in Stanford from 2006 to 2009.… In 2009 Dr.…
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Knowledge Transfer 13 Apr 2017
OnDA Solution Scattering enables real-time analysis of solution scattering data at XFELs.… Originally designed for serial crystallography by Valerio Mariani at CFEL, OnDA enables real-time analysis of experiments before data are even written to disk.… BioXFEL researcher Tom Grant and summer student Sarah…
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X-ray Data Collection Workshop
Events 16 Mar 2019
Mar 16 2019
BioXFEL, UPR, SSRL, and Rigaku are coordinating a scientific workshop for protein structure researchers in Puerto Rico on March 16th-17th, 2019. We will be offering a two-day workshop on X-ray data collection that provides hands-on training in X-ray data collection on a Rigaku home source and remotely at SSRL.… We will also…
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The three-dimensional structure of Drosophila melanogaster (6–4) photolyase at room temperature
BioXFEL Publications 29 Jul 2021 Contributor(s): suraj pandey, Emina Stojkovic, Marius Schmidt
http://dx.doi.org/10.1107/s2059798321005830 (6–4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is to repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster (6–4) photolyase [Dm(6–4)photolyase] at room and cryogenic temperatures are reported. The…
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BioXFEL Journal Club with Ahmad Hosseinizadeh (UWM) & Christopher Kuptiz (UWM)
Events 15 Jul 2015
Jul 15 2015
Ahmad Hosseinizadeh, a postdoc at the University of Wisconsin-Milwaukee, will present the article: … "Arnlund, D., Johansson, L. C., et al. (2014). Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser.
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BioXFEL Publications 21 May 2021 Contributor(s): Edward Snell
http://dx.doi.org/10.1093/icb/icab069 Abstract Many biological systems across scales of size and complexity exhibit a time-varying complex network structure that emerges and self-organizes as a result of interactions with the environment.… A wide range of networks exist in biology, from gene regulatory networks…
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BioXFEL Publications 2 May 2023 Contributor(s): Kara Zielinski, Andrea Katz, George Douglas Calvey, Lois Pollack
http://dx.doi.org/10.1107/s2052252523003482 Advances in time-resolved structural techniques, mainly in macromolecular crystallography and small-angle X-ray scattering (SAXS), allow for a detailed view of the dynamics of biological macromolecules and reactions between binding partners.… Most mix-and-inject approaches rely on…
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The accurate assessment of small-angle X-ray scattering data
BioXFEL Publications 24 Dec 2014 Contributor(s): Joseph Luft, Edward Snell
Small-angle X-ray scattering (SAXS) has grown in popularity in recent times with the advent of bright synchrotron X-ray sources, powerful computational resources and algorithms enabling the calculation of increasingly complex models.… Here, a series of metrics to quantitatively describe SAXS data in an objective manner using…
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Structural enzymology using X-ray free electron lasers
News 9 Mar 2017
Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse.… It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from…
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Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization
BioXFEL Publications 22 Nov 2024 Contributor(s): Tek Narsingh Malla, SRINIVASAN MUNIYAPPAN, Emina Stojkovic, Shibom Basu, Marius Schmidt
https://doi.org/10.1107/s2052252524010868 The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution.… Here, we report experiments emerging…
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Events 25 Jun 2019
Jun 25 2019
International Symposium on Structure Biology for Drug Discovery@SwissFEL With the first encouraging SwissFEL experiments in structural biology delivering static and time-resolved information on compound binding it is time to discuss the recent developments and shape the future directions.… The areas to be covered by the talks are…
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Publications 5 Dec 2014 Contributor(s): Tom Grant
Authors: Tenboer, J., Basu, S., Zatsepin, N., Pande, K.… , Frank, M., Hunter, M., Boutet, S., Williams, G. J., Koglin, J. E., Oberthuer, D., Heymann, M., Kupitz, C., Conrad, C., Coe, J., Roy-Chowdhury, S., Weierstall, U., James, D., Wang, D., Grant, T., Barty, A., Yefanov, O., Scales, J., Gati, C., Seuring, C., Srajer, V., Henning,…
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BioXFEL Publications 15 Nov 2016 Contributor(s): Garrett Charles Nelson, Chelsie E Conrad, Jesse David Coe, Yun Zhao, Robert Dods, Chufeng Li, Mark S. Hunter, Henry Chapman, Petra Fromme, Matthias Frank, Uwe Weierstall, John Spence, Gebhard F.X. Schertler
http://dx.doi.org/10.1107/s2053273316099368 Przemyslaw Nogly and Valerie Panneels and Garrett Nelson and Cornelius Gati and Tetsunari Kimura and Christopher Milne and Despina Milathianaki and Minoru Kubo and Wenting Wu and Chelsie Conrad and Jesse Coe and Richard Bean and Yun Zhao and Petra Bath and Robert Dods and Rajiv Harimoorthy and Kenneth…
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BioXFEL Publications 22 Aug 2016 Contributor(s): Garrett Charles Nelson, Chelsie E Conrad, Jesse David Coe, Yun Zhao, Robert Dods, Chufeng Li, Mark S. Hunter, Henry Chapman, Petra Fromme, Matthias Frank, Uwe Weierstall, John Spence, Gebhard F.X. Schertler
http://dx.doi.org/10.1038/ncomms12314 Przemyslaw Nogly and Valerie Panneels and Garrett Nelson and Cornelius Gati and Tetsunari Kimura and Christopher Milne and Despina Milathianaki and Minoru Kubo and Wenting Wu and Chelsie Conrad and Jesse Coe and Richard Bean and Yun Zhao and Petra Båth and Robert Dods and Rajiv Harimoorthy and Kenneth…
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BioXFEL Postdoctoral Research Award
Education 6 Sep 2016
Purpose: The BioXFEL STC has reserved discretionary funds intended to support a unique project for a postdoctoral scholar of the BioXFEL Center.… Our first fellowship was awarded to Michael Thompson.… Most recently, Andrea Katz was awarded in 2019.… Procedures: Funding requests must be…
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Members
James Fraser is an Assistant Professor in the Department of Bioengineering and Therapeutic Sciences at the University of California at San Francisco and the California Institute for Quantitative Biosciences (QB3).… Dr. Fraser completed his PhD with Tom Alber at UC Berkeley, where he studied protein structure and…
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Events 18 Oct 2018
Oct 18 2018
Dr. Martin Trebbin is Empire Innovation Assistant Professor at the Department of Chemistry and connected to the NSF science and technology center BioXFEL and RENEW.… This interdisciplinary research profile fits and aligns very with the mission and goals of RENEW and its members. Our world is dynamic and complex. This simple fact…
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Courses 13 Nov 2014
The BioXFEL Lecture Series is an online colloquium featuring lectures on various topics related to XFEL science in biology. Prominent experts in their respective fields, lecturers will present a broad range of materials from an introduction to XFEL radiation to more advanced topics of time-resolved studies, data analysis, single particle…
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Events 7 Feb 2017
Feb 07 2017
LaserLab Training School 2017 Deadline March 1, 2017 … Training School on Laser Applications for Biology and Biomolecular Systems: an authentic hands-on experience Dates 3-7 July 2017, Coimbra, Portugal The Training School on Laser Applications for Biology and Biomolecular Systems is focused on the application of…
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John Spence at 4th International Conference on Ultrafast Structural Dynamics
Events 5 Dec 2017
Dec 05 2017
The 4th International Conference on Ultrafast Structural Dynamics will be held in Trieste, Italy December 5-7, 2017.… ICUSD is a platform for discussing the latest methodological, theoretical and scientific developments aimed at understanding real-time structural changes in materials science, chemistry and…
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BioXFEL Publications 20 Mar 2015 Contributor(s): Rahel Woldeyes, Michael Thompson, James Holton, James Fraser
Determining the interconverting conformations of dynamic proteins in atomic detail is a major challenge for structural biology.… Here we compare the conformational ensembles of CypA by fixed-target X-ray free electron laser (XFEL) crystallography and multitemperature synchrotron crystallography. The…
