News

Single-shot determination of focused FEL wave fields using iterative phase retrieval

BioXFEL scientist Marc Messerschmidt, along with his colleagues, published a research article in OSA publishing.

Summary:

Determining fluctuations in focus properties is essential for many experiments at Self-Amplified-Spontaneous-Emission (SASE) based Free-Electron-Lasers (FELs), in particular for imaging single non-crystalline biological particles.

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Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase

BioXFEL scientist Vadim Cherezov, along with others, publishes research article in ScienceDirect.

Abstract:

The nicotinamide nucleotide transhydrogenase (TH) is an integral membrane enzyme that uses the proton-motive force to drive hydride transfer from NADH to NADP+ in bacteria and eukaryotes.

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Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody

Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs.

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FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images

A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues–Frank space, in which all crystal systems can be represented without singularities.

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Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature

Cytochrome c oxidase (CcO), the terminal enzyme in the electron transfer chain, translocates protons across the inner mitochondrial membrane by harnessing the free energy generated by the reduction of oxygen to water.

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New algorithms extract biological structure from limited data

Understanding the 3D molecular structure of important nano-objects like proteins and viruses is crucial in biology and medicine. With recent advances in X-ray technology, scientists can now collect diffraction images from individual particles, ultimately allowing researchers to visualize molecules at room temperature.

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