Critical Role of Water Molecules in Proton Translocation by the Membrane-Bound Transhydrogenase

BioXFEL scientist Vadim Cherezov, along with others, publishes research article in ScienceDirect.


The nicotinamide nucleotide transhydrogenase (TH) is an integral membrane enzyme that uses the proton-motive force to drive hydride transfer from NADH to NADP+ in bacteria and eukaryotes.


Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody

Monoclonal antibodies provide an attractive alternative to small-molecule therapies for a wide range of diseases. Given the importance of G protein-coupled receptors (GPCRs) as pharmaceutical targets, there has been an immense interest in developing therapeutic monoclonal antibodies that act on GPCRs.


FELIX: an algorithm for indexing multiple crystallites in X-ray free-electron laser snapshot diffraction images

A novel algorithm for indexing multiple crystals in snapshot X-ray diffraction images, especially suited for serial crystallography data, is presented. The algorithm, FELIX, utilizes a generalized parametrization of the Rodrigues–Frank space, in which all crystal systems can be represented without singularities.


Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature

Cytochrome c oxidase (CcO), the terminal enzyme in the electron transfer chain, translocates protons across the inner mitochondrial membrane by harnessing the free energy generated by the reduction of oxygen to water.


New algorithms extract biological structure from limited data

Understanding the 3D molecular structure of important nano-objects like proteins and viruses is crucial in biology and medicine. With recent advances in X-ray technology, scientists can now collect diffraction images from individual particles, ultimately allowing researchers to visualize molecules at room temperature.


Pilus Machine: July PDB Molecule of the Month

Bacteria live in complex ecologies, filled with competitors, environmental dangers, and defensive hosts. They face these challenges in many ways: by gathering together into tough biofilm communities, by sharing genetic information encoding useful proteins, and by picking up and moving when things look better elsewhere.